Journal: Cell

Article Title: A Genetically Defined Compartmentalized Striatal Direct Pathway for Negative Reinforcement

doi: 10.1016/j.cell.2020.08.032

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: ​ REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit polyclonal anti-MOR Immunostar 24216 Rabbit polyclonal anti-tyrosine hydroxylase Millipore AB152 Chicken polyclonal anti-GFP Aves Labs GFP1020 Rabbit polyclonal anti-RFP Rockland 600-401-379 Rabbit monoclonal anti-HA-Tag Cell Signaling 3724S Mouse monoclonal anti-Parvalbumin Millipore MAB1572 Rabbit polyclonal anti-Somatostatin-14 Peninsula Laboratories T-4103 Goat polyclonal anti-ChAT Millipore AB144P Bacterial and Virus Strains AAV8-Ef1a-fDIO-GCaMP6m Laboratory of Karl Deisseroth N/A AAVdj-hSyn-Cre OFF /Flp ON -hChR2(H134R)-eYFP Fenno et al., 2014 Addgene 55648 AAVdj-hSyn-Cre ON /Flp OFF -hChR2(H134R)-eYFP Fenno et al., 2014 Addgene 55646 AAV8-EF1a-fDIO-Cre-p2A-mCherry Laboratory of Karl Deisseroth N/A AAV5-Ef1a-DIO-hChR2(H134R)-eYFP UNC N/A AAVdj-EF1a-fDIO-eYFP UNC N/A AAV8-hSyn-DIO-KORD-IRES-Mcitrine UNC N/A AAV2/8-Ef1a-fDIO-TVA-mCherry Laboratory of Z. Josh Huang N/A retroAAV2-CBA-fDIO-Cre Vigene Biosciences N/A AAV9-CAGGS-Flex-mKate-T2A-TVA HHMI Janelia Research Campus N/A AAV9-CAGGS-Flex-mKate-T2A-N2c-G HHMI Janelia Research Campus N/A Rbv-CVS-N2c-dG-GFP HHMI Janelia Research Campus Addgene 73461 AAV8-hSyn-DIO-mCherry Addgene Addgene 50459 Experimental Models: Organisms/Strains Mouse: Tg(Drd1a-cre)FK150Gsat/Mmucd (the “D1-Cre” line) MMRRC RRID: MMRRC_029178-UCD Mouse: B6.FVB(Cg)-Tg(Adora2a-cre) KG139Gsat/Mmucd (the “A2A-Cre” line) MMRRC RRID: MMRRC_036158-UCD Mouse: Tshz1-2A-FlpO This study N/A Mouse: Frt-Stop-Frt-TdTomato He et al., 2016 N/A Mouse: Pdyn-IRES-Cre (B6.Cg-129S-Pdyn tm1.1(cre)Mjkr/LowlJ) The Jackson Laboratory JAX: 027958 Mouse: Ai14 (B6.Cg-Gt(ROSA) 26Sortm14(CAG-tdTomato)Hze/J) The Jackson Laboratory JAX: 007908 Software and Algorithms ImageJ (Fiji) software NIH https://fiji.sc/ MATLAB Mathworks https://www.mathworks.com/ GraphPad Prism 7 GraphPad Software https://www.graphpad.com/ Open in a separate window KEY RESOURCES TABLE Tshz1 labels a population of striatal direct pathway medium spiny neurons (dMSNs) Tshz1 -expressing ( Tshz1 + ) dMSNs are localized in the striosome Tshz1 + striosomal dMSNs represent punishment and drive negative reinforcement Pdyn labels another population of striosomal dMSNs mediating positive reinforcement

Techniques: Software

Journal: Cell

Article Title: Regulation of Energy Expenditure by Brainstem GABA Neurons

doi: 10.1016/j.cell.2019.05.048

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Genotypes/sources for mice used in the above studies are listed in the . table ft1 table-wrap mode="anchored" t5 caption a7 REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Chicken polyclonal anti-GFP Abcam ab13970; RRID: AB_300798 Rabbit polyclonal anti-c-Fos Synaptic Systems 226003; RRID: AB_2231974 Rabbit monoclonal anti-c-Fos Cell Signaling 2250S; RRID: AB_2247211 Rabbit polyclonal anti-RFP Rockland 600-401-379; RRID: AB_2209751 Rat monoclonal anti-mCherry (16D7) ThermoFisher {"type":"entrez-nucleotide","attrs":{"text":"M11217","term_id":"333925"}} M11217 ; RRID: AB_2536611 Chicken polyclonal anti-mCherry Abcam ab205402; RRID: AB_2722769 Chemicals, Peptides, and Recombinant Proteins Clozapine N-oxide Sigma-Aldrich C0832-5MG Critical Commercial Assays RNAscope Multiplex Fluorescent Assay V2 Advanced Cell Diagnostics (ACD Bio) 323100 RNAscope Probe- Mm-Slc32a1 ACD Bio 319191-C1 RNAscope Probe- mCherry-C2 ACD Bio 431201-C2 RNAscope Probe- EGFP ACD Bio 400281-C1 RNAscope Probe- tdTomato-C2 ACD Bio 317041-C2 Experimental Models: Organisms/Strains Mouse: Vgat-IRES-Cre: Slc32a1 tm2(cre)Lowl /J The Jackson Laboratory 016962; RRID: ISMR_JAX:016962 Mouse: Rosa26-LSL-EGFPL10a: B6.129S4- Gt(ROSA)26Sor tm1(CAG-EGFP/Rp/10a,birA)Wtp /J The Jackson Laboratory 022367; RRID: IMSR_JAX:022367 Mouse: C57BL/6J The Jackson Laboratory 000664; RRID: IMSR_JAX:000664 Mouse: TRAP2: Fos tm2.1(icre/ERT2)Luo /J The Jackson Laboratory 030323; RRID: IMSR_JAX:030323 Mouse: Ai14: B6;129S6- Gt(ROSA)26Sor tm14(CAG-tdTomato)/Hze /J The Jackson Laboratory 007908; RRID: IMSR_JAX:007908 Recombinant DNA AAV5-EF1a-DIO-hChR2(H134R)-EYFP UNC Vector Core N/A AAV5-EF1a-DIO-EYFP UNC Vector Core N/A AAV5-hSyn-DIO-hM3D(Gq)-mCherry Addgene 44361 AAV5-hSyn-DIO-hM4D(Gi)-mCherry Addgene 44362 AAV5-EF1a-fDIO-HA-NLS-Cre This paper; available at Addgene N/A AAV9-CAG-FLEX-EGFP; AI854 Penn Vector Core N/A AAV9-DIO-SynP-mCherry MGH Vector Core AAV-RN1 AAV9-DIO-SynP-EYFP MGH Vector Core AAV-RN2 PRV-lp298 (PRV-mCherry) This paper N/A PRV-152 (PRV-GFP) Smith et al., 2000 N/A Software and Algorithms GraphPad Prism 6.0 GraphPad N/A ClearMap Renier et al., 2016 N/A Imaris 9.0.1 Bitplane N/A Terastitcher University Campus Bio-Medico of Rome N/A Open in a separate window KEY RESOURCES TABLE

Techniques: Recombinant, Multiplex Assay, Fluorescence, Plasmid Preparation, Software